Fig. 6: Loss of 53BP1 and XLF causes distinct deletion patterns, and the effect of 53BP1 dominates in the double mutant. | Nature Communications

Fig. 6: Loss of 53BP1 and XLF causes distinct deletion patterns, and the effect of 53BP1 dominates in the double mutant.

From: 53BP1-RIF1 and DNA-PKcs show distinct genetic interactions with diverse chromosomal break repair outcomes

Fig. 6

A Combined loss of 53BP1 and XLF causes an increase in −2 and −7 deletions, similar to the loss of 53BP1 alone. Shown are deletion sizes for the samples shown in Fig. 5C. Frequencies represent the mean ± SD. n = 3 biologically independent transfections. Statistics with an unpaired t-test using Holm–Sidak correction. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. = not significant. § = deletion size was only found in 1 or 2 replicates, † = deletion size was not found in any of the replicates, and ‡ = deletion size was found in <2 Parental replicates. B Microhomology use varies with distinct deletions. Shown is the fraction of microhomology used for each deletion size and experimental condition shown in (Fig. 5C). n = 3 biologically independent transfections. Source data are provided as a Source data file.

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