Fig. 6: Genome assemblies for non-human vertebrates.

a Nx plot shows contig sizes sorted from largest to smallest, relative to cumulative assembly size, as a percentage of the haploid genome size for each species. From left to right the plots show genome assemblies for: Gould’s petrel (Pterodroma leucoptera); redstriped eartheater cichlid (Geophagus surinamensis); orange-bellied parrot (Neophema chrysogaster); western saw-shelled turtle (Myuchelys bellii; see Supplementary Note 3). The petrel and cichlid were assembled with PacBio HiFi (1 SMRT cell; base) plus ONT duplex data (2 duplex cells; cornetto). The parrot and turtle were assembled using ONT data, one or two standard flow cells (base) plus another with adaptive sequencing (Cornetto; simplex reads; LSK114). For ONT-only experiments, diploid assemblies were generated and haplotypes are plotted separately. b For the petrel and turtle assemblies above, bar plots show sizes of the fifty largest contigs in descending order, coloured according to presence of telomere sequences at contigs ends (both ends = purple; one end = pink). Equivalent plots for the cichlid and parrot are shown in Supplementary Fig. 5. c Bar plots show standard quality metrics for the same assemblies as in (a). From left to right, these are: contig N₅₀ lengths in Mbases; number of complete chromosomes (>1 Mbase and telomere detected at both ends); proportion of BUSCO genes detected as complete, duplicated, fragmented or missing. All plots show data for n = 1 assembly.