Fig. 4: VRK-1 depletion leads to the production of oocytes with aberrant chromosomes. | Nature Communications

Fig. 4: VRK-1 depletion leads to the production of oocytes with aberrant chromosomes.

From: BAF-1–VRK-1 mediated release of meiotic chromosomes from the nuclear periphery is important for genome integrity

Fig. 4

a Schematic representation showing the migration of transition zone nuclei after 50 h. b -1 oocytes stained with DAPI for the indicated genotypes and conditions. DAPI body numbers are indicated on the bottom left of each inset. Scale bar: 10 µm. c Quantification of DAPI bodies in the −1 oocyte for the indicated genotypes after 50 h treatment with ethanol or auxin. Mean ± SD per genotype (n= number of -1 oocytes): vrk-1::AID::ha, ethanol (n = 61): 6.0 ± 0.2, auxin (n = 70): 7.8 ± 1.6, P < 0.0001; vrk-1::AID::ha; spo-11 (ok79), ethanol (n = 30): 11.9 ± 0.3, auxin (n = 33): 11.9 ± 0.2, P > 0.9999; vrk-1::AID::ha; msh-5 (me23), ethanol (n = 26): 11.9 ± 0.5, auxin (n = 35): 11.7 ± 0.7, P = 0.5530; vrk-1::AID::ha auxin vs vrk-1::AID::ha; spo-11 (ok79) auxin and vrk-1::AID::ha auxin vs vrk-1::AID::ha; msh-5 (me23) auxin, P < 0.0001. The Two-sided Mann–Whitney test was used to assess statistical significance. d Number of chromosome bridges in the -1 oocyte for the indicated genotypes after 50 h treatment with ethanol or auxin. Mean ± SD per genotype (n= number of -1 oocytes): vrk-1::AID::ha, ethanol (n = 33): 0.2 ± 0.4, auxin (n = 46): 2.3 ± 1.3, P < 0.0001; vrk-1::AID::ha; spo-11 (ok79), ethanol (n = 30): 0.0 ± 0.0, auxin (n = 33): 0.0 ± 0.0, P > 0.9999; and vrk-1::AID::ha; msh-5 (me23), ethanol (n = 26): 0.2 ± 0.5, auxin (n = 35): 0.5 ± 1.1, P = 0.1761; vrk-1::AID::ha auxin vs vrk-1::AID::ha; spo-11 (ok79) auxin and vrk-1::AID::ha auxin vs vrk-1::AID::ha; msh-5 (me23) auxin, P < 0.0001. The Two-sided Mann–Whitney test was used to assess statistical significance. e Scheme depicting the localization of DTN-1::GFP. f DAPI (magenta) and GFP (yellow) stained -1 oocytes for the indicated genotype and conditions. Scale bar: 10 µm. g Schematic representations and representative examples of the different chromosome aberrations observed in vrk-1::AID::ha; dtn-1::gfp oocytes for the indicated conditions, showing numbers and calculated P values (Two-sided Fischer’s exact test). h Scheme depicting the migration of transition nuclei from L4 stage in 48 hours. i Number of chromosome bridges in the -1 oocyte for the indicated genotypes and conditions. Mean ± SD per genotype (n= number of -1 oocytes): vrk-1::AID::ha: L4440–ethanol (n = 25): 0.1 ± 0.3 vs L4440–auxin (n = 26): 2.2 ± 1.0, P < 0.0001; baf-1 RNAi–ethanol (n = 27): 0.3 ± 0.9 vs baf-1 RNAi–auxin (n = 37): 0.6 ± 0.7, P = 0.0147; L4440–ethanol vs baf-1 RNAi–auxin, P = 0.0004; and L4440–auxin vs baf-1 RNAi–auxin, P < 0.0001. The Two-sided Mann–Whitney test was used to assess statistical significance.

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