Fig. 5: Autophagy is essential for spermine-mediated αS degradation in C.elegans model of PD. | Nature Communications

Fig. 5: Autophagy is essential for spermine-mediated αS degradation in C.elegans model of PD.

From: Spermine modulation of Alzheimer’s Tau and Parkinson’s α-synuclein: implications for biomolecular condensation and neurodegeneration

Fig. 5

A Representative images of NL5901 worms treated with 500 µM of SPM (right) or vehicle control (left) and fed with empty vector (ev, top), sms RNAi (middle), smd RNAi (bottom). B Quantification of condensate of αS-YFP in NL5901 C.elegans that treated with empty vector, sms, smd RNAi, and SPM or vehicle control (n = 15). (RNAi ev: p = 0.0012, RNAi sms: p = 0.0001, RNAi smd: p = 0.0003). C Representative images of NL5901 worms treated with 500 µM of SPM (right) or vehicle control (left) and fed with atg-10 RNAi (top), lc3 RNAi (middle), and lamp RNAi (bottom). D Quantification of condensate of αS-YFP in NL5901 C.elegans that treated with empty vector, atg-10 RNAi, lc3 RNAi, lamp RNAi, and SPM or vehicle control (n = 15). (RNAi ev: p = 0.0012, RNAi atg10: p = 0.0085, RNAi lc3: p = 0.5304, RNAi lamp: p = 0.9324, Control: RNAi ev vs RNAi atg10, p < 0.0001; RNAi ev vs RNAi lc3, p < 0.0001; RNAi ev vs RNAi lamp, p = 0.0005;)The scale bar is: 20 µm. The data represent the mean ± SEM. A paired t-test was used in (C, D) (ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001).

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