Fig. 1: Upstream H3K36me3 activity and cryo-EM structure of the State 1 poised methylation complex.
From: Molecular mechanism of co-transcriptional H3K36 methylation by SETD2
a Schematic representation of nucleosome template and proteins used in (b) SETD2 deposits H3K36me3 on an upstream nucleosome. RNA extension by the methylation-competent elongation complex through mononucleosomes with extended run-up distance and T-less cassette to bp 32 (SHL − 4) into the nucleosome. RNA primer contains a 5’ Cy5 label. RNA lengths are indicated on the right side of the denaturing PAGE gel and ssRNA marker on the left. Western blot for H3K36me3 of RNA extension assay products. Assay performed in triplicates. Source data are provided as a Source Data file. c Overall structure of the State 1 complex with magnified view of FACT binding to upstream hexasome. d Alternate view of the State 1complex.
