Fig. 2: CXL-MS analysis poised methylation complex.
From: Molecular mechanism of co-transcriptional H3K36 methylation by SETD2
a Schematic overview of the RTF1 and SPT16 domain architecture. Interchain BS3 crosslinks are visualized between RTF1 and SPT16. Only crosslinks with a -log10Score > 1 are shown. Crosslinks to the N-terminal remnant of the RTF1 and SPT16 affinity tags are not displayed. Initial visualization of crosslinks performed in xiNET100. b Magnified view of FACT “fastener” binding SPT16. Interchain BS3 crosslinks are visualized. SPT16 visualized as coulombic surface potential. c Schematic overview of the SPT6 and SETD2 domain architecture. Interchain BS3 crosslinks are visualized between the proteins (d) overall structure of the complex. SPT6 residues that crosslink with SETD2 colored yellow. Magnified view of the SPT6 binding region of SETD2.
