Fig. 5: Non-membrane targeting mechanisms of AMP 1003. | Nature Communications

Fig. 5: Non-membrane targeting mechanisms of AMP 1003.

From: An antimicrobial peptide as a potential therapy for bacterial pneumonia that alleviates antimicrobial resistance

Fig. 5: Non-membrane targeting mechanisms of AMP 1003.

a DNA-binding ability of AMP 1003 to MRSA ATCC 33591 and K. pneumoniae ATCC 700603. n = 3 independent replicates. ROS accumulation of MRSA ATCC 33591 (b) and K. pneumoniae ATCC 700603 (c) treated with AMP 1003, n = 3 independent replicates. Data are presented as mean values ± SD. Data points show the individual values, the central line represents the median, and the error bars indicate the range. Statistical analysis: two-tailed t-test. Nucleic acid and proteins leak of MRSA ATCC 33591 (d, e) and K. pneumoniae ATCC 700603 (f, g) treated with AMP 1003, n = 3 independent replicates. Data are presented as mean values ± SD. Data points show the median, and the error bars indicate the range. Extracellular and intracellular ATP levels of MRSA ATCC 33591 (h, i) and K. pneumoniae ATCC 700603 (j, k) treated with AMP 1003, n = 3 independent replicates. Data are presented as mean values ± SD. Data points show the individual values, the central line represents the median, and the error bars indicate the range. Statistical analysis: two-tailed t-test. l Proposed antimicrobial mechanism of AMP 1003 against gram-positive and gram-negative bacteria. Source data are provided as a Source Data file.

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