Fig. 5: DNA damage recognition by DDB2 in the native nucleosome after UV irradiation.
From: Structural basis of cyclobutane pyrimidine dimer recognition by UV-DDB in the nucleosome
a Workflow of native nucleosome isolation from expi293 cells and production of native UV-irradiated nucleosomes. b Isolated nucleosomes from expi293 cells with or without UV irradiation (500 J/m2) were subjected to Native-PAGE or SDS-PAGE, and then stained with SYBR Gold (left panel) or Oriole (right panel). c Gel shift assay, performed using 40 nM of nucleosomes and UV-DDB (0–160 nM). All reactions were subjected to 6% Native-PAGE and DNA was stained by SYBR Gold. d Amounts of CPDs in nucleosomes with or without UV irradiation (500 J/m2) were measured by ELISA, using an anti-CPD antibody. The Y-axis represents the absorbance at 490 nm, normalized to the minimum value across all measurements, which was set to zero. The graph was generated using Prism 10 software, and all data are based on three technical replicates and are presented as mean ± s.d.
