Fig. 3: Signaling pathways differentially regulating function in each synaptic type from the trisynaptic circuit.

Signaling pathways overrepresented amongst DE proteins in each synapse of the trisynaptic loop. Pathways specific to CA3-CA1 synapses are framed in a blue box, pathways specific to DG-CA3 in a green box, those common to these 2 in a yellow box and, finally, pathways specific to EC-DG synapses are in an orange box. Relative protein abundance for each of the 9 samples investigated by LC-MS/MS are presented as z-scores in heatmaps. A title and a heatmap is presented for each overrepresented pathway. Related pathways (i.e. CA3-CA1 pathways involved in Energy Metabolism) are framed with a dashed black line. For some pathways (i.e. Traffic of A2-containing AMPARs), we also present a heatmap with proteins that have a clear DE but did not reach statistical significance (Not Significant). In the ‘Rhos Activate Wasps and Waves’ gene names of members of the Arp2/3 complex are in bold.