Fig. 1: Pol θ is dispensable for productive CSR. | Nature Communications

Fig. 1: Pol θ is dispensable for productive CSR.

From: Polymerase theta repairs persistent G1-induced DNA breaks in S-phase during class switch recombination

Fig. 1: Pol θ is dispensable for productive CSR.The alternative text for this image may have been generated using AI.

a Representative FACS plots of IgM and IgG1 expression in WT, Polq−/−, Xrcc4−/−, Xrcc4−/− Polq−/−, Shld1−/− and Shld1−/− Polq−/− primary B cells (CD19+), at day 4 post cytokine exposure (anti-IgD dextran/LPS/IL-4). b, c Quantifications of cells expressing IgG1+ (b) and of Ig-negative (IgM− IgG1−) (c) cells. Bars represent mean ± SEM, Two-tailed Mann-Whitney, WT (n = 8), Polq−/− (n = 11), Xrcc4−/− (n = 6), Xrcc4−/− Polq−/− (n = 5), Shld1−/− (n = 4) and Shld1−/− Polq−/− (n = 6), p values are indicated in Source Data file. d Cell trace violet (CTV) profiles in proliferating (anti-IgD dextran) (top) and IgG1 stimulating (bottom) conditions at day 3. e–g Percentage of switched cells at day 4 post cytokine exposure towards (e) IgG2b (anti-IgD dextran/LPS), WT (n = 8), Polq−/− (n = 11), Xrcc4−/− (n = 6), Xrcc4−/− Polq−/− (n = 5), Shld1−/− (n = 4) and Shld1−/− Polq−/− (n = 5), f IgG3 (anti-IgD dextran/LPS), WT (n = 8), Polq−/− (n = 11), Xrcc4−/− (n = 5), Xrcc4−/− Polq−/− (n = 5), Shld1−/− (n = 5) and Shld1−/− Polq−/− (n = 5) and g IgA (anti-IgD dextran/TGF-β/LPS/IL-5/RA), WT (n = 6), Polq−/− (n = 8), Xrcc4−/− (n = 4), Xrcc4−/− Polq−/− (n = 5), Shld1−/− (n = 4) and Shld1−/− Polq−/− (n = 5). Bars represent mean ± SEM, Two-tailed Mann-Whitney, p values are indicated in the Source Data file. Source data are provided as a Source Data file.

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