Fig. 4: Branched actin increases membrane tension.
From: Adherent cells sustain membrane tension gradients independently of migration
a Representative fluorescence images at bottom plane of HeLa cells on ring micropatterns stained with phalloidin (magenta) and vinculin antibody (cyan) under different drug treatments. b Top, average confocal FLIM images at the bottom plane of HeLa cells labeled with Flipper-TR under different drug treatments. Bottom, average Flipper-TR lifetime as a function of the distance from center of micropattern. Shaded areas represent adhesive micropattern location. c Top, flipper-TR lifetime difference respective to JLY treatment. Bottom, flipper-TR lifetime difference respective to JLY treatment as a function of the distance from center of micropattern. d Average Flipper-TR lifetime (ns) per cell of cross (green), crossbow (blue), and ring (orange) micropatterned cells under different drug treatments. Labels represent Welch’s test P value (P < 10−3) and difference from control in ns. Data distribution in black. e Quantification of flipper-TR lifetime gradient: average lifetime in ‘high region’ (center of the micropattern) and ‘low region’ (inner edge of micropattern) under different drug treatments. a–c: Scale bar, 10 µm. d,e N as marked per condition in panel b. Boxplots show the median (center line), 25th and 75th percentiles (bounds of the box), and whiskers extending to the most extreme data points within 1.5 × the interquartile range from the box.
