Fig. 5: Spatial distribution of lipid composition in patterned cells.
From: Adherent cells sustain membrane tension gradients independently of migration
a Schematics representing MALDI-MSI procedure. b Histogram of pixel-wise lipid fractions, and average lipid fractions for different lipid species: sphingomyelin (SM, magenta), ceramide (Cer, gray), phosphatidylethanolamine (PE, blue), phosphatidylcholine (PC, orange), and globotriaosylceramide (Gb3, green). c Cell averaging of spatial MALDI-MSI of different lipid species. Color scale represents local differences from mean value normalized in terms of standard deviations. Arrows indicate observed lipid accumulation. d Average fluorescence images at basal plane of HeLa cells in cross, crossbow, and ring (n > 20 each) micropatterns stained with filipin, Cholera toxin, Equina toxin, and Shiga toxin targeting cholesterol, GM1, sphingomyelin, and globotriaosylceramide, respectively, under control and JLY treatment. Scale bar, 10 µm. e, f Average fluorescence intensity profile of filipin (B, cholesterol) and Cholera toxin (C, GM1) across the line marked in panel D for control and JLY treatment.
