Fig. 2: Map-based cloning and functional analysis of GmSMS6.
From: A 14-3-3 modulator of seed weight and quality for unlocking the yield potential of soybean
a GmSMS6 was mapped to the qSMS6 region on chromosome 2. b Fine-mapping of GmSMS6 to a 308.5-kb region. The green and blue bars represent the homozygous alleles from Zp661 and sms6, respectively. The bar graph on the right indicates 100-seed weight variation for each recombinant. GmSMS6 is highlighted in the red box, and the black boxes indicate the other genes within this genomic region. c 100-seed weight (n = 4 individuals) and indicated seed traits (n = 40 seeds) for Zp661, CR-SMS6Z1, and CR-SMS6Z2. d Comparison of seed length among Jack and indicated CR-SMS6 lines. Bar = 10 mm. e 100-seed weight (n = 10 individuals) and indicated seed traits (n = 60 seeds) in Jack and CR-SMS6 lines. f Comparison of seed length among Jack, OE-SMS6G, and OE-SMS6A, where OE-SMS6G lines. g 100-seed weight (n = 10, 4, 4, 4, and 4 individuals for Jack, OE1-SMS6G, OE2-SMS6G, OE1-SMS6A, and OE2-SMS6A lines, respectively) and indicated seed traits (n = 60, 40, 40, 40, and 40 seeds for the same lines) in Jack and four OE-SMS6 lines. h Scanning electron microscopy (SEM) analysis of the mature seed cotyledons (inner surface) from Jack, CR1-SMS6, OE1-SMS6G, and OE1-SMS6A lines. Bar = 10 µm (cells) and 2 mm (cotyledons). i Cell area, cotyledon area, and cell number in mature seeds (n = 4) of Jack, CR1-SMS6, OE1-SMS6G, and OE1-SMS6A plants. The cell size (n = 406, 457, 432, and 508 cells for each genotype, respectively) were measured. Data are shown as means ± SD. In all the box plots, the center line indicates the median, the edges of the box represent the first and third quartiles, and the whiskers extend to the smallest and largest data points within 1.5 interquartile ranges from the edges. Statistically significant differences (c, e) were determined by two-sided Student’s t-tests. *** p < 0.001. The different lowercase letters (g, i) indicate statistically significant differences (p < 0.05) determined by two-sided one-way ANOVA tests with Duncan’s multiple range tests. ns, not significant. Source data are provided as a Source Data file.
