Fig. 6: Comparison of the survival rate of the parental line, the selected strain, and clinical isolates with MQ resistance, in response to PPQ with or without MQ pre-incubation.

Means and standard deviations are shown on the graphs; each dot represents an independent replicate. P-values are based on two-sided tests. The median survival of parasites to 200 nM PPQ is unchanged in presence of MQ pre-incubation before exposure to PPQ in the parental strain (p = 0.4688, Wilcoxon matched-pairs signed rank test, n = 6 biolocal replicates, grey). The pre-incubation with MQ significantly increased the survival of the selected strain (** p = 0.0038, paired t-test, n = 7 biological replicates, pink) and of clinical MQ-R parasites (** p = 0.0024, Wilcoxon matched-pairs signed rank test, n = 12 biological replicates, yellow). Shapiro-Wilk test p-value were 0.0290, 0.2313, 0.2989, 0.4944, 0.0095, and 0.0054, respectively for Parental 200 nM PPQ, Parental 200 nM MQ + 200 nM PPQ, Pressure 4 200 nM PPQ, Pressure 4 200 nM MQ + 200 nM PPQ, Clinical MQ-R isolates 200 nM PPQ, and Clinical MQ-R isolates 200 nM MQ + 200 nM PPQ. Zeros are shown as 0.001 for log scale purposes. Source data are provided as a Source Data file. ns: non significant.