Fig. 2: The G308S mutant TUBB protein shows reduced incorporation into microtubules. | Nature Communications

Fig. 2: The G308S mutant TUBB protein shows reduced incorporation into microtubules.

From: Mutations in the β-tubulin TUBB impair ciliogenesis and are associated with ciliopathy-like phenotypes

Fig. 2: The G308S mutant TUBB protein shows reduced incorporation into microtubules.The alternative text for this image may have been generated using AI.

a Representative images of hTERT-RPE1 cells overexpressing wild-type or G308S TUBB-V5 and immunostained for V5 (green) and total α-tubulin (red). [i, ii, iii] Magnifications of the indicated areas in the merged images (white boxes) showing complete microtubule incorporation [i], diffuse distribution [ii] and partial microtubule incorporation [iii] of TUBB-V5. b Percentage of cells overexpressing wild-type or G308S TUBB-V5 (V5-positive) showing complete microtubule incorporation, partial microtubule incorporation, or diffuse distribution of TUBB-V5 as determined by the appearance of the V5-signal. Data from three experiments (n = 133 cells for TUBB wild-type and n = 126 cells for TUBB G308S) are represented as mean ± standard deviation. c Scatter plot showing TUBB-V5 colocalization with α-tubulin, quantified using thresholded Mander’s coefficient (n = 54 cells for TUBB wild-type and n = 35 cells for TUBB G308S, from three experiments). Data are represented as mean ± standard error of the mean. P-value was calculated using an unpaired two-tailed t-test. Source data are provided as a Source Data file.

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