Fig. 5: Intermittent YAP activation rescues dendritic and bone defects in the Piezo1-deficient mice. | Nature Communications

Fig. 5: Intermittent YAP activation rescues dendritic and bone defects in the Piezo1-deficient mice.

From: Piezo1-mediated mechanotransduction controls osteocyte maturation and dendrite development via a YAP-CCN-Src signaling axis

Fig. 5: Intermittent YAP activation rescues dendritic and bone defects in the Piezo1-deficient mice.The alternative text for this image may have been generated using AI.

a Phalloidin staining (green; nucleus: blue) of dendritic network, quantified in Supplementary Fig. 6c (N = 3 biological replicates), and b silver nitrate staining of LCN, quantified in Supplementary Fig. 6d (N = 3 biological replicates), in Dmp1Cre; TetOYAP* (YAP gain) mice subjected to constant (cons.) or intermittent (inter.) YAP activation for 5-weeks. Mice were collected at 8-weeks-old. c, d µCT analysis of femur cortical and trabecular bone in YAP gain mice. N = 5 biological replicates. e Second harmonic generation (SHG, white) imaging of femur midshaft cortical bone in WT and YAP gain mice. N = 3 biological replicates. f Dynamic histomorphometry analysis of femur midshaft cortical bone, quantified in Supplementary Fig. 6f. N = 5 biological replicates. Calcein (green; nucleus: blue) was injected at 8 and 2 days before collection at 8-week-old. g–j IF staining of SPP1, DMP1, PHEX, and MMP13 (cyan; nucleus: blue) in WT and YAP Gain mice, quantified in Supplementary Fig. 6i, j. N = 3 biological replicates. k, l µCT analysis of femur cortical and trabecular bone in WT and Piezo1 cKO mice treated with YAP gain. Mice were treated with intermittent Dox for 5 weeks and collected at 12-weeks old. N = 9 (WT, Piezo1 cKO and Piezo1 cKO with YAP gain) and 6 (WT with YAP gain) biological replicates. m Phalloidin (green; nucleus: blue) and silver nitrate staining of femur cortical midshaft in WT, Piezo1 cKO, and Piezo1 cKO with YAP gain mice, quantified in Supplementary Fig. 6o, r. N = 3 biological replicates. Scale bar: a,b, e–j, m 50 µm, c, k 100 µm. Bar graphs represent mean ± SD. d one-way ANOVA with Holm-Sidak’s post-hoc correction. k, l Two-way ANOVA using Piezo1 cKO and YAP gain as independent variables. Holm-Sidak’s post-hoc correction was performed to compare group differences. Source data are provided as a Source Data file.

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