Fig. 6: Wnt inhibition rescues dendritic defects in the Piezo1-deficient mice.
a Schematic of low-dose LGK974 treatment and Axin2 expression measured by qRT-PCR analysis of bone tissue 6 h after LGK974 treatment. N = 4 (Vehicle and 1 mg/kg LGK974) and three (2 mg/kg LGK974) biological replicates. b qRT-PCR analysis of osteocyte (Dmp1, Phex) and osteoblast (Sp7, Spp1) marker genes in cortical bone tissue 6 h after 2 mg/kg LGK974 treatment. N = 4 biological replicates. c Phalloidin staining (green; nucleus: blue) and osteocyte dendrite quantification of femur midshaft cortical bone in WT and Piezo1 cKO mice treated with LGK974 for 5 weeks. Mice were collected at 8-weeks old. N = 3 biological replicates. d Phalloidin staining (green; nucleus: blue) and osteocyte dendrite quantification of femur midshaft cortical bone in WT and Piezo1 cKO mice with 1 copy of β-catenin deletion (Ctnnb1f/+). N = 3 biological replicates. e, f µCT analysis of femur cortical and trabecular bone in LGK974-treated WT and Piezo1 cKO mice. N = 5 (WT, Piezo1 cKO, Piezo1 cKO treat with LGK974) and 6 (WT treated with LGK974) biological replicates. g, h µCT analysis of femur cortical and trabecular bone in 8-week-old WT and Piezo1 cKO mice with Ctnnb1f/+. N = 5 (WT) and 4 (Piezo1 cKO, Ctnnb1 HET-KO, and Piezo1 cKO with Ctnnb1 HET-KO) biological replicates. Scale bar: c, d 50 µm, e, g 200 µm. Bar graphs represent mean ± SD. a one-way ANOVA with Holm-Sidak’s post-hoc correction. b Two-sided unpaired Student’s t test. c, d, f, h Two-way ANOVA using Piezo1 cKO and LGK974/Ctnnb1f/+ as independent variables. Holm-Sidak’s post-hoc correction was performed to compare group differences. Source data are provided as a Source Data file.
