Fig. 7: Piezo1 promotes dendrite formation via activation of the YAP-CCN1/2-Src signaling axis. | Nature Communications

Fig. 7: Piezo1 promotes dendrite formation via activation of the YAP-CCN1/2-Src signaling axis.

From: Piezo1-mediated mechanotransduction controls osteocyte maturation and dendrite development via a YAP-CCN-Src signaling axis

Fig. 7: Piezo1 promotes dendrite formation via activation of the YAP-CCN1/2-Src signaling axis.The alternative text for this image may have been generated using AI.

a Schematic of OCY454 cells in 3D hydrogels co-cultured with Huh-7 cells. Created in BioRender. Hu, Y. (2025) https://BioRender.com/4k88lcq. b Phalloidin staining (green; nucleus: blue) of WT OCY454 cells co-cultured with GFP/CCN1/CCN2-expressing Huh-7 cells for 5 days, quantified in Supplementary Fig. 8a. N = 5 independent experiments. c Phalloidin staining (green; nucleus: blue) of Piezo1-/- OCY454 cells co-cultured with Huh-7 cells overexpressing CCN1 and CCN2 for 5 days, quantified in Supplementary Fig. 8b. N = 5 independent experiments. d WB analysis of pSrc and Src levels in WT OCY454 cells treated with 1 µM Yoda1 and 10 µM BAPTA-AM for 24 h. Experiment was repeated three times with similar results. Representative data from 1 experiment is shown. e WB analysis of pSrc, Src, pYAP and YAP levels in WT OCY454 cells after 6-h treatment with conditioned media from Huh-7 cells overexpressing CCN1/CCN2 and 100 nM dasatinib. Experiment was repeated three times with similar results. Representative data from one experiment is shown. f Phalloidin staining (green; nucleus: blue) and dendrite quantification of WT and Piezo1-/- OCY454 cells treated with 100 nM dasatinib for 3 days. N = 3 independent experiments. g Phalloidin staining (green; nucleus: blue) and osteocyte dendrite quantification of WT and Piezo1-/- OCY454 cells overexpressing SrcY527F cultured for 3 days. N = 3 independent experiments. h, i Phalloidin staining (green; nucleus: blue) and osteocyte dendrite quantification of WT and Piezo1-/- OCY454 cells co-cultured with Huh-7 cells overexpressing CCN1 and CCN2 treated with 100 nM Dasatinib for 3 days. N = 3 independent experiments. j WB analysis of pYAP, YAP, TAZ, pSrc, and Src levels in WT OCY454 cells treated with 100 nM Dasatinib or 300 nM of TDI-011536 for the indicated durations. Experiment was repeated three times with similar results. Representative data from one experiment is shown. Bar graphs represent mean ± SD. Scale bar: (b, c, f–h)100 µm. f, g, i one-way ANOVA with Holm-Sidak’s post-hoc correction. Source data are provided as a Source Data file.

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