Fig. 6: GDP-L-fucose induces ABCB1 transcription via c-Jun to enhance the expression of Caco2 cells. | Nature Communications

Fig. 6: GDP-L-fucose induces ABCB1 transcription via c-Jun to enhance the expression of Caco2 cells.

From: Odoribacter splanchnicus rescues aging-related intestinal P-glycoprotein damage via GDP-L-fucose secretion

Fig. 6

A, B Whole RNA-seq analysis of control Caco2 cells (PBS) or cells exposed for 48 h to GDP-L-fucose (250 μM), or to O.splanchnicus (OS) supernatant (n  =  5/group). A TPM levels of ABCB1 in the three groups. B Spearman’s correlation analysis between the expression of ABCB1 and each of the transcription factors (TFs) known or predicted to have inductive, variable, or repressive effects on ABCB1 expression. C Predicted c-Jun binding ABCB1 promoter motifs by UCSC Genome Browser Home and PROMO. D, E The binding of ABCB1 promoter and Histone H3, IgG, and c-Jun was further explored by ChIP-PCR (D) and ChIP-qPCR (E) assays in Caco2 (n  = 6/group). F–H Caco2 cells were transfected with si-NC or si-JUN (1) for 48 h (n  =  6/group). F, G Expression levels of P-gp and c-Jun (F) and relative quantification (G) in Caco2 cells. H ABCB1 and JUN mRNA levels in Caco2 cells. I–K Caco2 cells were transfected with si-NC or si-JUN (1) and with or without GDP-L-fucose (250 μM) for 48 h (n  =  6/group). I, J Expression levels of P-gp and c-Jun (I) and relative quantification (J) in Caco2 cells. K ABCB1 and JUN mRNA levels in Caco2 cells. Data are presented as mean ± SD. Statistical analyses were conducted using one-way ANOVA (A) and two-way ANOVA (G, H, J, K). Exact P values are reported in the figures.

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