Fig. 3: Supraphysiological concentrations of AMH impair the reproductive hormone profile of female domestic cats.

Female domestic cats were treated prepubertally with either 5 × 10¹² viral particles per kilogram of body weight of AAV9-Empty vector (Controls – CTL, black) or 5 × 10¹² (Low-dose – LOW, blue) or 1 × 10¹³ (High-dose – HIGH, red) viral genomes per kilogram of body weight of AAV9-fcMISv2. a Average circulating inhibin B concentrations in serum samples from 2 to 26 months post-treatment were compared to the average concentrations in the control group. Average estrogen b and progestogen c metabolite levels in fecal samples collected three times weekly after the age of 8 months until the end of the study in both groups of treated females were compared to the average levels in the control group. d Average circulating luteinizing hormone concentrations in serum samples collected monthly from 2 to 12 months post-treatment were compared to the average concentrations in the control group. Log-transformed data were compared using a one-way ANOVA (Dunnett’s post-hoc test): *P = 0.0151 for inhibin B, ****P < 0.0001 for fecal estrogens and progestogens, *P = 0.0144 for fecal progestogens *P = 0.0292 (low-dose) and *P = 0.0149 (high-dose) for luteinizing hormone. n  =  2, 4, and 3 female domestic cats in the control, low-dose, and high-dose groups, respectively. Repeated measurements collected monthly a, d or three times per week b, c are shown for each cat. Data are shown as means ± SD. Source data are provided as a Source data file.