Fig. 1: The cryo-EM structure of Pra1 reveals a hexameric assembly.
From: Structural insights into mechanisms of zinc scavenging by the Candida albicans zincophore Pra1
a The cryo-EM map of C. albicans Pra1. b The hexamer consists of an assembly of a trimer of dimers. A dimer is shown in split pea green (subunit E) and forest green (subunit F). Each Pra1 subunit is related to its neighbor by a 180° rotation, resulting in two distinct interaction interfaces per subunit. Subunits E + F, A + B, and C + D make up the dimers. c The N-terminus of a Pra1 subunit forms a brace or arm that is held across part of its neighboring subunit to form a dimer. Here, the N-terminus of subunit E is shown forming a brace across part of subunit F. The N-terminal brace intra-dimer interactions are primarily mediated by salt bridges and hydrogen bonds. d Apart from the N-terminal brace interactions, Q188 and S93 form polar interactions across the intra-dimer interface. e, f The interactions at the inter-dimer interface are polar in nature.
