Fig. 5: Improvement of mRNA stability and translation activity by sugar and backbone modifications. | Nature Communications

Fig. 5: Improvement of mRNA stability and translation activity by sugar and backbone modifications.

From: Position-specific ORF nucleoside-ribose modifications enabled by complete chemical synthesis enhance mRNA stability and translation

Fig. 5: Improvement of mRNA stability and translation activity by sugar and backbone modifications.The alternative text for this image may have been generated using AI.

A Design and sequence of chemically synthesized mRNAs and in vitro-transcribed mRNAs. B Time course of concentration of remaining RNA after incubating with diluted mouse serum (n = 3 biological replicates). C, D Time course of concentration of encoded peptide from HeLa cells after lipofection using messengerMAX (n = 4 biological replicates) (C) and electroporation (D) of each mRNA (n = 4 biological replicates). The data were represented as the mean. The statistically significant differences for each mRNA from “NK041” (B–D) in one-way ANOVA followed by Dunnett’s test were marked as follows. n.s., p > 0.05; *p  <  0.05; **p  <  0.01; ***p  <  0.001. Source data are provided as a Source Data file.

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