Fig. 1: Crystal structure of hcis-PT in the apo state.
From: Structural mechanisms of allosteric regulation in the human cis-prenyltransferase complex
Cartoon representation of the crystallographic asymmetric unit, composed of a DHDDS (blue) and NgBR (yellow) heterodimer. Active site secondary structure elements of interest are labeled. The dashed rectangle frames the NgBR C-terminus and the DHDDS diphosphate binding region, shown in the zoom perspective on the right. The holo structure, with bound FsPP and IPP (PDB 7PAX), is shown for comparison. Note that in the apo state, the NgBR C-terminus as well as the side chains of the catalytic residues R38 and R85 could not be resolved, due to enhanced flexibility. In addition, a distinct salt bridge between R37 and D34 of DHDDS is formed.
