Fig. 5: Inter-subunit allosteric communication paths perturbations alter hcis-PT function.
From: Structural mechanisms of allosteric regulation in the human cis-prenyltransferase complex
a Network analysis of allosteric communication path trajectories. Originating from NgBRS249 (green sphere), the paths connecting the predicted network (red spheres) and catalytic (cyan spheres) residues are highlighted (arrows). Note that in some cases, network and catalytic residues overlap. b Average time-dependent increase in MANT-O-GPP fluorescence in the presence of hcis-PT harboring DHDDSWT versus the indicated allosteric path mutants. Data are presented as mean ± SEM. c The initial slope of the fluorescence curves, reflecting the initial reaction rate, is presented as mean ± SEM (One-way ANOVA followed by Dunnett’s multiple comparisons test; * P = 0.04, *** P < 0.001, **** P < 0.0001. For all comparisons, n = 44; measurements for each construct were obtained from three biological replicates). Source data are provided as a Source Data file.
