Fig. 4: LUBAC and TRAF6 modulate MALT1 substrate recognition.
From: LUBAC modulates CBM complex functions downstream of TRAF6 in T cells
a Detection of catalytically active MALT1 in parental, TRAF6 KO, HOIP KO, TRAF6/HOIP DKO or HOIL-1 KO Jurkat T cells under untreated or P/I stimulated conditions (30 min) by pulldown of biotin-MALT1-ABP. Substrate cleavage was quantified by determining the ratio of active MALT1 (MALT1 ABP-PD) to total MALT1 (lysate) from 3 biological replicates. b–d Cleavage of MALT1 substrates was detected in parental, as well as two independent clones of TRAF6 KO, HOIP KO, TRAF6/HOIP DKO or HOIL-1 KO Jurkat T cells, either untreated or P/I stimulated (30 min) by Western blot. For quantification the ratios of cleavage products to full-length proteins were determined for HOIL-1 (b), CYLD (c), and N4BP1 (d) from 3 biological replicates. All bars represent the means ± SEM and p-values were calculated by one-way ANOVA combined with Dunnett’s multiple comparisons test. Only significant p-values (<0.05) are shown. kDa kilodalton.
