Fig. 2: Data driven analysis corroborates distinct type-III specific phenotype of platelets.

a Mean fluorescence intensities (MFI) of TLR2, CD154 and CD32 across different timepoints. b Quantification of selected clusters at indicated timepoints across healthy controls (control), psoriasis (pso), and atopic dermatitis patients (AD). Statistical analysis was carried out using one-sided unpaired student’s t tests. Depicted p values under 0.05 are considered significant with a Benjamini-Hochberg (BH) false discovery rate (FDR) <5%. c Follow-up of CD32⁺CD154⁺ platelet frequencies from baseline to one month (T1) and four months (T4) post systemic therapy initiation. d Uniform Manifold Approximation and Projection (UMAP), subsequently clustered using FlowSOM, of psoriasis patient platelets over baseline, T1, and T4. e Individual correlation of CD32⁺CD154⁺ platelet frequencies (logit transformed) with psoriasis area and severity index (PASI) in psoriasis patients. Data shown in (a–e) represents the analysis of cohort 1 (n = 10 controls, n = 27 psoriasis patients, n = 4 AD patients), which was processed, measured and analyzed in one batch. f Frequency of CD32⁺CD154⁺ platelets in the validation cohort 2, which was processed, measured, and analyzed in a separate second batch (n = 6 controls, n = 6 psoriasis patients, n = 10 AD patients). Statistical analysis was carried out using one-sided unpaired student’s t tests. Depicted p values under 0.05 are considered significant. g Summarizing overview of findings in this study, Created in BioRender. Heikenwälder, M. (2025) https://BioRender.com/5m1qd4y. Source data are provided in a source data file. For all depicted box plots in this figure, the horizontal line within each box indicates the median, while the lower and upper box edges denote the first and third quartiles, respectively. Whiskers extend to 1.5 times the interquartile range, and dots represent individual data points.