Fig. 4: Structural comparison of CA lattice and CA-CTD dimer between HTLV-1 CA crystals and retroviral subtomogram averages.
From: The Human T-cell Leukemia Virus capsid protein is a potential drug target
The crystal structures of the full-length HTLV-1 CA with 6-fold crystallographic symmetry (a) and the CACTD dimer (i) are presented in the left column. The subtomogram averages shown for comparison include the immature HTLV-1 CA (b and j; PDB 8PUG, 8PUH), and immature/mature HIV-1 (c, d, k and l; PDB 4USN, 5MCX, 5L93), Rous Sarcoma Virus (e, f, m and n; RSV; PDB 5A9E, 7NO2) as well as Murine Leukaemia Virus (g, h, o and p; MLV; PDB 6HWW, 6HWX). In structures derived from cryo-ET with subtomogram averaging, CANTD is shown in cyan, CACTD in gold, and the N-terminal β-hairpin connected to α-helix 1 in magenta. The residues of K18 in HTLV-1, R18 in HIV-1, and R21 in RSV are highlighted in navy. j–p depict the inter-hexamer CTD dimer mediated by helix 9 (green) with the corresponding dihedral angle shown below. Positive dihedral angles denote that the left-hand helix 9 is positioned behind its partner on the right-hand molecule. “N. D.” stands for no data.
