Fig. 1: The Vav gene is essential for dendritic filopodia outgrowth. | Nature Communications

Fig. 1: The Vav gene is essential for dendritic filopodia outgrowth.

From: The Vap33 signaling axis precisely coordinates the timing of motoneuron dendritogenesis in neural map development

Fig. 1

a A representative image of the aCC motoneuron (green) in abdominal segment 4 (A4) of an embryo at 15:00 after egg laying (AEL), shown in a ventral view with anterior oriented upwards. b Left: Representative images of dendritic outgrowth at 15:00 AEL in wild-type embryos, Vav knockout (Vav-/-), and Vav-/- or wild-type embryos with aCC-specific Vav expression. Images are oriented with anterior upwards and dorsal to the right. Dots mark the tips of dendritic filopodia longer than 1 μm. Right: Quantification of dendritic filopodia number for each genotype. Statistical significance was determined by one-way ANOVA (F 3,72 = 31, p = 4.2 × 10-13, followed by Tukey’s multiple comparison test. “ns” indicates p > 0.05, indicating no significant difference. A total of 20, 19, 19, and 18 neurons were analyzed for wild type (wt), Vav-/-, Vav in Vav-/-, and Vav in wt, respectively. Analyses of dendritic filopodia in all other figures were also performed at 15:00 AEL. Error bars represent the mean ± SEM. Scale bars: 100 μm in (a); 5 μm in (b). Source data are provided as a Source Data file.

Back to article page