Fig. 6: The effect of varying forces and superhelical densities on the activity of ANKLE1.
From: ANKLE1 processes chromatin bridges by cleaving mechanically stressed DNA
a Graph showing the fraction of uncleaved positively supercoiled DNA (σ = 0.1) over time. n = 23 and 19 for DNA under higher and lower forces, respectively. b Graph showing the fraction of uncleaved non-supercoiled DNA over times. n = 32 and 10 for DNA under higher and lower forces, respectively. c Table summarizing the half-lives of uncleaved DNA when incubated with ANKLE1. The half-lives were calculated by fitting the data to an exponential decay function (see Supplementary Fig. 7). d Bar graph showing the average cleavage time of negatively supercoiled DNA (σ = −0.1) under the indicated forces. The number of beads that detached from the surface is indicated in the graph. Error bars represent the mean ± SEM. e A bar graph showing the percentage of beads detached from the surface as in (d). The number of total beads examined is indicated in the graph. f Bar graph showing the average cleavage time of negatively supercoiled DNA (σ = −0.025, −0.05, −0.075, −0.1) under a tension of 5 pN. The number of beads that detached from the surface is indicated in the graph. Error bars represent the mean ± SEM. g Bar graph showing the percentage of beads detached from the surface as in (f). The number of total beads examined is indicated in the graph. h Model of how ANKLE1 is involved in resolving stretching chromatin bridges. See text for the details. Source data are provided as a Source Data file.
