Fig. 7: The most expanded BCR clones in kidney of lupus mice are highly mutated.

A Rank-abundance distribution plot of clonotypes displays the relative abundance (%) of clones in descending order across all tissues and conditions. Data points for each organ are color-coded: BM_healthy (green), spleen_healthy (purple), BM_sick (light green), spleen_sick (light purple) and kidney_sick (orange). B Pie charts depicting the percentage of shared clonal populations among BM, spleen, and kidney tissues under healthy and diseased conditions. Each chart illustrates the extent of clonal overlap among tissues, as well as the proportion of unique clones. C Heatmap shows the abundance of the top 10 expanded clones within each BM, spleen, and kidney tissues in a diseased state across different clusters. Each row represents a unique clone identified by its clone ID, and each column represents a specific cluster (0–12). None of the top clones were present in cluster 13. Color intensity indicates the log1p-transformed abundance within each cluster. Heatmap showing the abundance of the top clones across the different conditions and tissues (BM_healthy, spleen_healthy, BM_sick, spleen_sick, kidney_sick). Color intensity indicates log1p-transformed abundance. D Heatmap showing distribution of the abundance of Ig isotype per clone. Color intensity indicates log1p-transformed abundance. E Heatmap showing the median mutation frequency of the top clones across the different clusters. Color intensity represents the median mutation frequency, highlighting areas with higher mutation rates. F Heatmap illustrating the mean expression levels of Cd19 of the top clones across different clusters (0–12). None of the top clones were present in cluster 13. Color intensity indicates mean expression of Cd19. Experiments and analyses were performed with Sle123 mice. Data show compilation of n = 6 mice/group (BM and Spleen) and n = 13 mice (Kidney).