Fig. 5: AOX-MR abrogates apoptosis in activated T cells.

Splenic T cells were stimulated for 72 h with plate bound anti-CD3 and anti-CD28 antibodies as above. A Representative contour plot of viability analysis in CD8⁺ and CD4⁺ T cells using Live/Dead and Annexin V stains. B Quantification of viability analysis (N = 3–6/condition). C Caspase 3 (left), caspase 8 (center), and caspase 9 (right) activity in CD8⁺ and CD4⁺ T cells (N = 6–9/condition). Top, representative density plot; bottom, quantification of normalized mean fluorescence intensity (MFI). D Fas expression in CD8⁺ and CD4⁺ T cells (N = 10–15/condition). Left, representative density plot; right, quantification of normalized MFI. E FasL expression in CD8⁺ and CD4⁺ T cells (N = 10–15/condition). Left, representative density plot; right, quantification of normalized MFI. Cells were isolated by magnetic beads and stimulated for 72 h (for A) and 24 h for (B–E) with anti-CD3 and anti-CD28 in complete media. Data are representative of two to three independent experiments and indicate mean and standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001 by one-way ANOVA and post-hoc Dunnett test against WT.