Fig. 6: Effect of D580R mutation on current inhibition by intracellular Mg²⁺.

a, b On the left, the average voltage dependence of whole-cell currents recorded from HEK 293 cells expressing wild-type hTRPV6 (a) and D580R (b) in the absence or presence of different concentrations of intracellular Mg²⁺ using the voltage ramp protocol illustrated in Fig. 1a. The data for WT currents at 0 and 2 mM Mg²⁺ were taken from Fig. 1b, the data for D580R at 2 mM Mg²⁺ were taken from Fig. 5a. On the right, the current amplitudes normalized by cell size (mean ± SEM) measured at +80 mV using the currents on the left. P-values were determined using the Kruskal–Wallis test with multiple comparison corrections by Benjamini, Krieger, and Yekutieli test. Source data are provided. c, d Initial tail current amplitudes (mean ± SEM) measured for wild-type TRPV6 (c) and D580R (d) after returning to –100 mV during the voltage step protocol illustrated in Fig. 1c at different concentrations of intracellular Mg²⁺. n is the number of cells examined.