Fig. 1: Formate production and isotopic tracer analysis of methanol-dependent strain ΔfrmAΔtpiA. | Nature Communications

Fig. 1: Formate production and isotopic tracer analysis of methanol-dependent strain ΔfrmAΔtpiA.

From: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy

Fig. 1

a Optical density (OD) and formate production of the methanol-dependent strain ΔfrmAΔtpiA expressing mdh, hps, and phi during growth on methanol (500 mM) and pyruvate (20 mM) in shake flasks (n = 3). The mean of the data is shown as solid line and the shaded regions represent the range of the 95% confidence intervals. b Simplified metabolic scheme illustrating methanol-dependent growth of E. coli ΔfrmAΔtpiA expressing mdh, hps, and phi when cultivated on methanol and pyruvate. Molecules originating from formaldehyde or close to the one-carbon entry point are expected to be formed from methanol (green) while others are formed from pyruvate (orange). The canonical formaldehyde detoxification pathway of E. coli, which should not be active in the methanol-dependent strain due to its frmA deletion, is indicated in gray. c Fraction of formate molecules determined in the supernatant of the methanol-dependent strain originating from 13C methanol (green) and 12C pyruvate (orange) when sampled at stationary phase. R1-R4 indicate replicate experiments. Source data are provided as a Source Data file.

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