Fig. 4: Mdh Bm MGA3 does not catalyze the overoxidation of methanol to formate. | Nature Communications

Fig. 4: Mdh Bm MGA3 does not catalyze the overoxidation of methanol to formate.

From: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy

Fig. 4

In vitro assays of Mdh Bm MGA3 showing the initial change in NADH concentration by monitoring the absorption at 340 nm. Reactions contained 12.5 μM enzyme, 5 mM NAD+ and were started by the addition of 500 mM formaldehyde (a) or methanol (b), respectively. The mean of the data is shown as solid line and the shaded regions represent the range of the 90% confidence intervals. c Rate of NADH formation in the presence of 10, 20, 50, 100, and 500 mM methanol or formaldehyde. Methanol to formaldehyde reactions are indicated in blue, formaldehyde to formate in red. Experiments were performed in triplicates and data are represented as mean ± the standard deviation. Source data are provided as a Source Data file.

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