Fig. 2: Distinct transcriptome in epithelial colonoids from active UC pediatric patients. | Nature Communications

Fig. 2: Distinct transcriptome in epithelial colonoids from active UC pediatric patients.

From: Patient-derived colon epithelial organoids reveal lipid-related metabolic dysfunction in pediatric ulcerative colitis

Fig. 2: Distinct transcriptome in epithelial colonoids from active UC pediatric patients.

Pediatric epithelial colonoids differentiated for 3 days and were subjected to bulk RNA-seq. n = 8 donors/group. A Volcano plot of differentially expressed genes between aUC and C samples (Log2FC > 1, FDR < 0.1). The full list of differentially expressed genes is provided in Supplementary Data 1. B Heat map of the top 10 dysregulated genes showing the diversity and consistency of expression among each colonoid line. C Chemokine genes overexpressed in aUC epithelial colonoids compared to C in the bulk RNA-seq data (Log2FC > 1, FDR < 0.1). D Extracellular chemokine protein, CCL2, E CXCL11, and F CXCL1 after a 3-day differentiation. Each symbol represents duplicate measures for one donor. n = 4 donors/group. G Schematic of chemotaxis assay. Partly created in BioRender. Ojo, B. (2025) https://BioRender.com/nwwqemfH Neutrophil chemotaxis after 2 hours with conditioned medium. Each symbol represent the average duplicate measures for one donor. n = 4 donors/group. Statistics for D–F and H: two-sided unpaired t-test. For D–F, and H, boxplot represents the first, second (median), and third quartiles with whiskers representing the minimum and maximum points. P values are indicated in the figures.

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