Fig. 3: Multiple DARE cells predominantly appear at 24 hpi and extensively proliferate over the following 24 h to regenerate the WID. | Nature Communications

Fig. 3: Multiple DARE cells predominantly appear at 24 hpi and extensively proliferate over the following 24 h to regenerate the WID.

From: Apoptosis-resistant cells drive compensatory proliferation via cell-autonomous and non-autonomous functions of the initiator caspase Dronc

Fig. 3

a Schematic of the DDBS reporter combined with the G-TRACE system. Created in BioRender. Braun, T. & Yacobi-Sharon, K. (2025) https://BioRender.com/s6ebqxl. b WIDs expressing the transgenic combination described in (a) and monitored at the indicated time points after irradiation. c, d Quantification of total DARE cell fluorescent area shown in bii-iv (c) and bi, ii and fi, ii (d) expressed as a percentage of total WID area. e Adult wing shortly after eclosion derived from a 48 hpi larva (as in biv) displaying residual DARE cells (green). f Irradiated WIDs, as in (b), expressing the actDrice transgene in the DARE cells. g Quantification of total DARE cell fluorescent area shown in biv and fiv expressed as a percentage of total WID area. h Quantification of the total WID sizes (in square micrometers) in 48 hpi WT (biv) and actDrice-expressing DARE cells at 48 (fiv), 72, and 96 hpi. Statistical tests: One-way ANOVA followed by Tukey’s multiple comparisons test (c, h); two-tailed unpaired Student’s t-test (d, g). All data points, including outliers, are shown in box plot format. The lower and upper whiskers represent the minimum and maximum values, respectively; the center line denotes the median; and the lower and upper box bounds correspond to the medians of the lower and upper halves of the dataset. Each dot represents a single WID, reflecting the number of biologically independent WID samples (n). Numbers (n) of WIDs examined were: 202 (4 hpi), 178 (24 hpi), and 224 (48 hpi) in (c); 9 (WT non-irradiated), 11 (WT 4 hpi), 6 (actDrice non-irradiated), and 11 (actDrice 4 hpi) in (d); 27 (WT) and 20 (actDrice) in (g); and 27 (WT 48 hpi), 20 (actDrice 48 hpi), 6 (actDrice 72 hpi), and 4 (actDrice 96 hpi) in (h). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. p-values in (c): P < 0.0001 (4 hpi vs. 24 hpi), p < 0.0001 (24 hpi vs. 48 hpi), and p < 0.0001 (4 hpi vs. 48 hpi); (d): p = 0.0333 (WT non-irrad. vs. WT 4 hpi) and p = 0.0181 (actDrice non-irrad. vs. actDrice 4 hpi); (g): p < 0.0001 (WT vs. actDrice); (h): p = 0.0002 (WT 48 hpi vs. actDrice 48 hpi), p = 0.9559 (actDrice 48 hpi vs. actDrice 72 hpi), P = 0.0658 (actDrice 72 hpi vs. actDrice 96 hpi), and p = 0.0064 (actDrice 48 hpi vs. actDrice 96 hpi).

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