Fig. 8: Cell cycle-compromised DARE cells drive NARE cell overproliferation, restoring tissue size but impairing organ differentiation.

a, b Irradiated WIDs containing the _DDBS reporter, QUAS-Gal4 adapter, and UAS-RedStinger, with (b) or without (a) a UAS-Dap-GFP transgenic construct, were monitored at 4, 24, and 48 hpi. DARE cells are in red. c Quantification of total DARE cell fluorescent area shown in (a, b) expressed as a percentage of total WID area. d The graphs depict total size in square micrometers of WIDs shown in (a, b). e–g Overproliferation of WID NARE cells at the expense of DARE cells results in increased differentiation (e) and size (f, g) abnormalities in the adult wing. The graphs in (f) depict total size in square micrometers of adult wings shown in (g).Statistical tests: Two-tailed unpaired Student’s t-test (c, d, f). All data points, including outliers, are shown in box plot format. The lower and upper whiskers represent the minimum and maximum values, respectively; the center line denotes the median; and the lower and upper box bounds correspond to the medians of the lower and upper halves of the dataset. Each dot represents a single WID, reflecting the number of biologically independent WIDs samples (n). Numbers of WIDs examined were: 6 (WT 4 hpi), 14 (Dap^OE 4 hpi), 16 (WT 24 hpi), 17 (Dap^OE 24 hpi), 23 (WT 48 hpi), and 25 (Dap^OE 48 hpi) in (c, d). Numbers of wings examined were: 24 (WT females 4 hpi), 29 (Dap^OE females 4 hpi), 18 (WT males 4 hpi), 34 (Dap^OE males 4 hpi), 16 (WT females 24 hpi), 46 (Dap^OE females 24 hpi), 25 (WT males 24 hpi), 20 (Dap^OE males 24 hpi), 30 (WT females 48 hpi), 31 (Dap^OE females 48 hpi), 23 (WT males 48 hpi), and 26 (Dap^OE males 48 hpi). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. p-values in (c): P = 0.2373 (WT 4 hpi vs. Dap^OE 4 hpi), p = 0.0001 (WT 24 hpi vs. Dap^OE 24 hpi), and p < 0.0001 (WT 48 hpi vs. Dap^OE 48 hpi); (d): P = 0.3189 (WT 4 hpi vs. Dap^OE 4 hpi), p = 0.0425 (WT 24 hpi vs. Dap^OE 24 hpi), and p = 0.1067 (WT 48 hpi vs. Dap^OE 48 hpi); (f): p < 0.0001 (WT females 4 hpi vs. Dap^OE females 4 hpi), p = 0.0012 (WT males 4 hpi vs. Dap^OE males 4 hpi), p = 0.2935 (WT females 24 hpi vs. Dap^OE females 24 hpi), p = 0.5727 (WT males 24 hpi vs. Dap^OE males 24 hpi), p < 0.0001 (WT females 48 hpi vs. Dap^OE females 48 hpi), and p = 0.0078 (WT males 48 hpi vs. Dap^OE males 48 hpi). Black and red asterisks represent significant decrease or increase, respectively.