Fig. 2: Plasticity of the extracellular restriction site.
From: Structural basis of PANX1 permeation and positive modulation by mefloquine
a, b Constricted (a) and dilated (b) conformations of the extracellular restriction site, obtained through ECD-focused classification of the merged dataset (see Results, “W74 plasticity adjusts extracellular pore entrance”). Structures are viewed from the extracellular side. Cryo-EM maps are shown as transparent envelopes. PANX1 atomic models are displayed in cartoon representation, with the side chains of W74 and R75 shown as sticks. c, d Close-up views of the extracellular restriction site in the constricted (c) and dilated (d) conformations. The upper panels show extracellular views, with interactions between W74 and R75 indicated by dashed lines and their distances labeled. The angle between adjacent W74 side chains in the constricted conformation and the pore diameters in both conformations are indicated. The lower panels show views parallel to the membrane, with the long axes of the W74 side chains indicated by double-headed arrows.
