Fig. 4: Direct cytocompatibility assays with L929 fibroblast cells using fluorescence imaging with live/dead stains.
From: Thermo-reversible gelation of self-assembled conducting polymer colloids
Live cells fluoresce green (calcein-AM) while nuclei from dead cells fluoresce red (ethidium homodimer-1). a Live cell % for 3.6 wt% TR-CP in PBS (pH = 7) compared to controls. The cells were incubated for 96 h for each group. Data is presented as mean ± std. deviation of biological replicates. n = 3 for control, 2 for positive control, 4 for 3.6 wt% TR-CP. b Control: cells after incubating with control collagen gel; c 3.6% TR-CP: cells after incubating with TR-CP. d Live cell % for TR-CP/alginate composite. The cells were incubated for 48 h for each group. Data is presented as mean ± std. deviation. n = 3 for alginate, 3 for positive control, 5 for 3.6 wt% TR-CP, 2 for 5.6 wt% TR-CP. e Control: cells after incubating with control alginate gel; f 3.6 wt% TR-CP/alginate: cells after incubating with 3.6% TR-CP in alginate gel.
