Fig. 5: Spatial heterogeneity of mitochondria is general in cells.
From: Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes
a-d, FLIM imaging and phasor plot of PKMDR-stained mitochondria in MII oocyte (a), zygote (b), senescent HUVEC (c) and neuron (d). Plots (c, d) showing correlation between mitochondrial fluorescence lifetimes and their distance to the center of cell. R-values were calculated using Spearman correlation test. e, f Intensity and FLIM images of PKMDR-stained mitochondria in neuron. Plots (f) showing the average fluorescence lifetime observed in neurite and soma (n = 6 cells). P-values were calculated using paired two-tailed Student’s t-test. Scale bars, 20 μm.
