Fig. 6: Time-lapse FLIM recording reveals the temporal heterogeneity of mitochondria.
From: Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes
a Time-lapse FLIM imaging of PKMDR-stained mitochondria in L929 cells. Images were acquired every 15 s. Spontaneous mitochondrial membrane potential change events were indicated with arrows. b Fluorescence lifetime profiles of the two ROI specified in (a). c Time-lapse FLIM imaging of mitochondria in a neuron. Images were acquired every 11 s. Two mitochondria underwent transportation are indicated with arrows. Scale bars, 20 μm.
