Fig. 2: FL7 positively regulates plant osmotic stress response.

a Germination phenotypes. Data are means ± SD of three biological replicates, with each replicate containing at least 30 seedlings (two-way ANOVA with Tukey’s test; P < 0.05). b Stomatal aperture of plant treated with ABA. Epidermal strips with fully open stomata were treated with or without 20 µM ABA for 2 h before being photographed (left). Stomatal aperture (the ratio of width B to length A marked in red in figure) was quantified from each genotype (right). Data are means ± SD (two-way ANOVA with Tukey’s test; P < 0.05). Experiments were performed three times with similar results. c Drought phenotypes of Col-0, fl7 and OE-FL7 lines in soil. Data represent means ± SD from three biological replicates, with each replicate containing at least 20 plants (two-way ANOVA with Tukey’s test; P < 0.05). d Overlaps of DEGs from indicated lines treated with mannitol. The numbers accounted for genes shared by different subsets. e Heat map of mean-centred Z-scores for osmotic response marker genes arranged following k-means clustering. Box plot indicates the relative expression level based on median centred Z-score of osmotic response marker genes (right) (Data are means ± SD, n = 20, one-way ANOVA with Tukey’s test; P < 0.05). f Electrolyte leakage rate of plants treated with NaCl. Four-week-old plants were irrigated with saline water for a week. Data are means ± SD of three technical replicates (two-way ANOVA with Tukey’s test; P < 0.05). Experiments were performed three times with similar results. g The P. capsici inoculation phenotypes. Data are means ± SD (n = 12 leaves per group; *P < 0.05, **P < 0.01, Student’s t test, two-sided, ns not significant). The experiments were performed two times with similar results. Scale bar = 1 cm. Source data are provided as a Source Data file.