Fig. 3: Genetic analysis of fl7 with the pp2c-As mutants.
From: FL7 is an ancient ABA-independent inhibitor of PP2C-As regulating plant stress responses
a Germination phenotypes of abi1 hab1 pp2ca and abi1 hab1 pp2ca fl7 lines. The seeds were germinated on 1/2 MS medium with or without ABA for 7 days. Data are means ± SD of three biological replicates, with each replicate containing at least 30 seedlings (two-way ANOVA with Tukey’s test, P < 0.05). b Root growth phenotypes of Col-0, fl7, abi1 hab1 pp2ca and abi1 hab1 pp2ca fl7 lines treated with ABA. Seeds were germinated on 1/2 MS medium and then transferred to 1/2 MS medium containing 12 µM ABA and vertically grown for additional 5 days. The length of root was measured with Image J. Data are mean ± SD (n = 5; two-way ANOVA with Tukey’s test, P < 0.05). Scale bar = 0.5 cm. The experiments were performed three times with similar results. c Drought tolerance of Col-0, fl7, abi1 hab1 pp2ca and abi1 hab1 pp2ca fl7 lines. Data represent means ± SD from three biological replicates, with each replicate containing at least 20 plants (two-way ANOVA with Tukey’s test, P < 0.05). Source data are provided as a Source Data file.
