Fig. 3: Disruption of PG formation in E. faecium.
From: Peptidoglycan recruitment by a penicillin binding protein
a Expected result of the disk assay using E. faecium strain without PBP5 (D344SRF, ΔPBP5). b Disk diffusion assay. 1, E. faecium D344SRF vs. ceftriaxone; 2, E. faecium D344SRF + PBP5 plasmid vs. ceftriaxone; 3, E. faecium D344SRF + empty plasmid vs. ceftriaxone; 4, E. faecium D344SRF + PBP5_V1 plasmid vs. ceftriaxone; 5, E. faecium D344SRF + PBP5_V2 plasmid vs. ceftriaxone; 6, E. faecium D344SRF + PBP5_V3 plasmid vs. ceftriaxone; 7, E. faecium D344SRF + PBP5_V4 plasmid vs. ceftriaxone; 8, E. faecium D344SRF + PBP5_V5 plasmid vs. ceftriaxone; 9, E. faecium D344SRF + PBP5_V6 plasmid vs. ceftriaxone. c Western Blot for PBP5 protein expression. Lane numbers correspond to experiments in (b).; blot was performed n = 1. d PBP5 variants used in disk assay mapped on PBP5 surface (gray surface; PDBid: 6MKA). Orange: V1 and V2, Cyan: V3, Green: V4, Magenta: V5 and Blue: V6.
