Table 2 Genotype of flies in each figure

From: Differential modulation of feedforward inhibition reflects topographic organization in the olfactory system

Figure

Genotype

Purpose

Fig. 1a

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;;5-HT2B-T2A-GAL4MI05208/TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT2B T2A Gal4 and 5-HT2B T2A LexA driver lines

Fig. 1b

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;5-HT1A-T2A-GAL4MI04464;TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT1A T2A Gal4 and 5-HT2B T2A LexA driver lines

Fig. 1c

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;;5-HT7-GAL4MI00215/TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT7 T2A Gal4 and 5-HT2B T2A LexA driver lines

S. Fig. 1b

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;;Mi{Trojan-lexA:QFAD.2}Gad1[MI09277-TlexA:QFAD.2]/+

Depiction of fascicle formed by primary neurites of GABAergic v-PNs as they enter the AL

S. Fig. 1c

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;;Mi{Trojan-lexA:QFAD.0}ChAT[MI04508-TlexA:QFAD.0]/TM3

Depiction of fascicle formed by primary neurites of cholinergic lv-PNs as they enter the AL

S. Fig. 1g,l

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;5-HT1A-T2A-GAL4MI04464;TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT1A T2A Gal4 and 5-HT2B T2A LexA driver lines

S. Fig. 1h, m

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;5-HT1B-T2A-GAL4MI05213;TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT1B T2A Gal4 and 5-HT2B T2A LexA driver lines

S. Fig. 1i, n

w*;lexAop RFP-UAS GFP; 5-HT2A-T2A-GAL4MI00459/TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT2A T2A Gal4 and 5-HT2B T2A LexA driver lines

S. Fig. 1j, o

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;;5-HT2B-T2A-GAL4MI05208/TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT2B T2A Gal4 and 5-HT2B T2A LexA driver lines

S. Fig. 1k, p

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;;5-HT7-GAL4MI00215/TI{2A-lexA::GAD}5-HT2B

Intersection of v-PNs included in the 5-HT7 T2A Gal4 and 5-HT2B T2A LexA driver lines

S. Fig. 1q, u

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;5-HT1A-T2A-GAL4MI04464;TI{2A-lexA::GAD}5-HT7

Intersection of v-PNs included in the 5-HT1A T2A Gal4 and 5-HT7 T2A LexA driver lines

S. Fig. 1r, v

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;5-HT1B-T2A-GAL4MI05213;TI{2A-lexA::GAD}5-HT7

Intersection of v-PNs included in the 5-HT1B T2A Gal4 and 5-HT7 T2A LexA driver lines

S. Fig. 1s, w

w*;lexAop RFP-UAS GFP; 5-HT2A-T2A-GAL4MI00459/TI{2A-lexA::GAD}5-HT7

Intersection of v-PNs included in the 5-HT2A T2A Gal4 and 5-HT7 T2A LexA driver lines

S. Fig. 1t,x

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;;5-HT7-GAL4MI00215/TI{2A-lexA::GAD}5-HT7

Intersection of v-PNs included in the 5-HT7 T2A Gal4 and 5-HT7 T2A LexA driver lines

S. Fig. 2a

hs-FLPG5.PEST/yw;5-HT1B-T2A-GAL4MI05213;10xUAS(FRT.stop)myr::smGdP-HA}VK00005,10xUAS(FRT.stop)myr::smGdP-V5-THS-,10xUAS(FRT.stop)myr::smGdP-FLAG

Example AL and LH of clones from MCFO of 5-HT1B expressing v-PNs

S. Fig. 2b

hs-FLPG5.PEST/yw;;10xUAS(FRT.stop)myr::smGdP-HA}VK00005,10xUAS(FRT.stop)myr::smGdP-V5-THS-,10xUAS(FRT.stop)myr::smGdP-FLAG/5-HT2A-T2A-GAL4MI00459

Example AL and LH of clones from MCFO of 5-HT2A expressing v-PNs

S. Fig. 2c

hs-FLPG5.PEST/yw;;10xUAS(FRT.stop)myr::smGdP-HA}VK00005,10xUAS(FRT.stop)myr::smGdP-V5-THS-,10xUAS(FRT.stop)myr::smGdP-FLAG/5-HT7-GAL4MI00215

Example AL and LH of clones from MCFO of 5-HT7 expressing v-PNs

Fig. 3a, b

w*/yw;UAS-C3PA-GFP/5-HT1A-T2A-GAL4MI04464; + /TM6B

Projection patterns observed after photoactivation of v-PNs expressing the 5-HT1A receptor

Fig. 3c

w*/yw;UAS-C3PA-GFP/5-HT1B-T2A-GAL4MI05213; + /TM6B

Projection patterns observed after photoactivation of v-PNs expressing the 5-HT1B receptor

Fig. 3d

w*/yw;UAS-C3PA-GFP/ + ;5-HT7-GAL4MI00215/TM6B

Projection patterns observed after photoactivation of v-PNs expressing the 5-HT7 receptor

Fig. 4a

w*;;R24H08-GAL4/40XUAS-IVS-mCD8::GFP

Morphology of R24H08 v-PNs in the AL and LH

Fig. 4b

w*;;R86G06-GAL4/40XUAS-IVS-mCD8::GFP

Morphology of R86G06 v-PNs in the AL and LH

Fig. 4e

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R24H08-LexA/5-HT1A-T2A-GAL4MI04464

Intersection of v-PNs included in the 5-HT1A T2A Gal4 and R24H08-LexA

Fig. 4f

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R24H08-LexA/5-HT1B-T2A-GAL4MI05213

Intersection of v-PNs included in the 5-HT1B T2A Gal4 and R24H08-LexA

Fig. 4g

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R86G06-LexA;5-HT7-T2A-GAL4MI00215

Intersection of v-PNs included in the 5-HT7 T2A Gal4 and R86G06-LexA

Fig. 4i, j

w*;10XUAS-IVS-mCD8::GFP;R24H08-GAL4

R24H08-Gal4 stably expressing GFP for HCR labeling against the 5-HT1A and 5-HT1B receptors

Fig. 4k

w*;10XUAS-IVS-mCD8::GFP;R86G06-GAL4

R86G06-Gal4 stably expressing GFP for HCR labeling against the 5-HT7 receptors

S. Fig. 3a, b

w*;R24H08-AD;VT046303-DBD/40XUAS-IVS-mCD8::GFP

Validation of splitGal4 for v-PNs in R24H08

S. Fig. 3c

w*;R24H08-AD/lexAop RFP-UAS GFP;VT046303-DBD/GAD1-Trojan-LexA

Verifying R24H08 v-PNs express GAD1

S. Fig. 3d

w*;lexAop RFP-UAS GFP; R86G06-LexA/GAD1-Trojan-LexA

Verifying R86G06 v-PNs express GAD1

S. Fig. 3e, f

w*;R24H08-LexA/lexAop RFP-UAS GFP;Mz699-Gal4

Verifying R24H08 v-PNs are included in Mz699 v-PNs

S. Fig. 3g, h

w*;R86G06-LexA/lexAop RFP-UAS GFP;Mz699-Gal4

Verifying R86G06 v-PNs are included in Mz699 v-PNs

S. Fig. 3i

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R24H08-LexA/5-HT1A-T2A-GAL4MI04464

Intersection of v-PNs included in the 5-HT1A T2A Gal4 and R24H08-LexA

S. Fig. 3j

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R24H08-LexA/5-HT1B-T2A-GAL4MI05213

Intersection of v-PNs included in the 5-HT1B T2A Gal4 and R24H08-LexA

S. Fig. 3k

w*;R24H08-LexA/lexAop RFP-UAS GFP;5-HT2A-T2A-GAL4MI00459

Intersection of v-PNs included in the 5-HT2A T2A Gal4 and R24H08-LexA

S. Fig. 3l

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R24H08-LexA/5-HT2B-T2A-GAL4MI05208

Intersection of v-PNs included in the 5-HT2B T2A Gal4 and R24H08-LexA

S. Fig. 3m

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R24H08-LexA;5-HT7-T2A-GAL4MI00215

Intersection of v-PNs included in the 5-HT7 T2A Gal4 and R24H08-LexA

S. Fig. 3n

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R86G06-LexA/5-HT1A-T2A-GAL4MI04464;

Intersection of v-PNs included in the 5-HT1A T2A Gal4 and R86G06-LexA

S. Fig. 3o

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R86G06-LexA/5-HT1B-T2A-GAL4MI05213;

Intersection of v-PNs included in the 5-HT1B T2A Gal4 and R86G06-LexA

S. Fig. 3p

w*;R86G06-LexA/lexAop RFP-UAS GFP;5-HT2A-T2A-GAL4MI00459

Intersection of v-PNs included in the 5-HT2A T2A Gal4 and R86G06-LexA

S. Fig. 3q

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R86G06-LexA/5-HT2B-T2A-GAL4MI05208

Intersection of v-PNs included in the 5-HT2B T2A Gal4 and R86G06-LexA

S. Fig. 3r

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R86G06-LexA;5-HT7-T2A-GAL4MI00215

Intersection of v-PNs included in the 5-HT7 T2A Gal4 and R86G06-LexA

Fig. 5a, b

w*;;R86G06-GAL4/20XUAS-IVS-jGCaMP7f

w*;R24H08-AD/ + ;VT046303-DBD/20XUAS-IVS-jGCaMP7f

Ca2+ imaging of odor responses from R24H08 and R86G06 v-PNs

Fig. 5c, d

w*;R86G06-LexA/UAS-CsChr;R60F02-Gal4/lexAop-jGCaMP7f

Ca2+ imaging of odor responses from R86G06 v-PNs with or without preceding optogenetic activation of the CSDns via R60F02.

Fig. 5e, f

w*;R24H08-LexA/UAS-CsChr;R60F02-Gal4/lexAop-jGCaMP7f

Ca2+ imaging of odor responses from R24H08 v-PNs with or without preceding optogenetic activation of the CSDns via R60F02.

S. Fig. 4a, b

w*;+/UAS-CsChr;R60F02-Gal4/UAS-jGCaMP7f

Ca2+ imaging of optogenetically stimulated activation of CSDns

S. Fig. 4c, d

w*;R86G06-LexA/UAS-CsChr;R60F02-Gal4/lexAop-jGCaMP7f

Ca2+ imaging of R86G06 v-PNs with optogenetic activation of CSDns

S. Fig. 4e, f

w*;R24H08-LexA/UAS-CsChr;R60F02-Gal4/lexAop-jGCaMP7f

Ca2+ imaging of R24H08 v-PNs with optogenetic activation of CSDns

Fig. 6a, b

w*;+/20XUAS-IVS-jGCaMP7f;R86G06-Gal4/+

Ca2+ imaging of odor responses from R86G06 v-PNs before and after application of Serotonin(5-HT) and AS-19

Fig. 6c

w*;+/20XUAS-IVS-jGCaMP7f;R86G06-GAL4/UAS-5-HT7RNAi

Ca2+ imaging of odor responses from R86G06 v-PNs while driving 5HT7 receptor RNAi

Fig. 6d, e

w*;R24H08-AD/20XUAS-IVS-jGCaMP7f;VT046303-DBD

Ca2+ imaging of odor responses from R24H08 v-PNs before and after application of Serotonin(5-HT) and Ipsapirone

Fig. 6f

w*;R24H08-AD/20XUAS-IVS-jGCaMP7f;VT046303-DBD/UAS-5-HT1aRNAi

Ca2+ imaging of odor responses from R24H08 v-PNs while driving 5HT1A receptor RNAi

S. Fig. 5a, b

w*;+/20XUAS-IVS-jGCaMP7f;R86G06-GAL4/+

Ca2+ imaging of odor responses from R86G06 v-PNs before and after application of Metitipine and SB-269970

S. Fig. 5c–f

w*;R24H08-AD/20XUAS-IVS-jGCaMP7f;VT046303-DBD/+

Ca2+ imaging of odor responses from R24H08 v-PNs before and after application of Serotonin (5-HT), Ipsapirone, Metitipine, WAY100635

S. Fig. 5g

w*;20XUAS-IVS-jGCaMP7f/+;R86G06-GAL4/+

w*;R24H08-AD/20XUAS-IVS-jGCaMP7;VT046303-DBD/+

Baseline Ca2+ change of R24H08 and R86G06 v-PNs and bath application of Ipsapirone and AS-19

S. Fig. 5h, i

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R24H08-LexA/+;R60F02-Gal4/+

Depiction of overlap in projection of R24H08 v-PNs and the CSDns in the AL and LH

S. Fig. 5j

w*,10XUAS-IVS-mCD8::RFP,13XLexAop2-mCD8::GFP;R86G06-LexA/+;R60F02-Gal4/+

Depiction of overlap in projection of R86G06 v-PNs and the CSDns in the LH

Fig. 7a

w*;lexAop-rCD2::RFP-p10.UAS-mCD8::GFP-p10/R24H08-LexA;R86G06-GAL4

Comparing morphology of R24H08 and R86G06 v-PNs

Back to article page