Fig. 7: Subpopulation analysis and cell communication analysis of gliocyte. | Nature Communications

Fig. 7: Subpopulation analysis and cell communication analysis of gliocyte.

From: Mid-gestational cell-type-specific transcriptomic signatures in the prefrontal and superior temporal cortex in Down syndrome

Fig. 7: Subpopulation analysis and cell communication analysis of gliocyte.The alternative text for this image may have been generated using AI.

a Visualization of OPC subclusters using UMAP. b Dot plot showing the average expression of marker gene of OPC subclusters. c Bar plot showing the proportions of OPC subclusters in the control and DS from PFC and STP. d Representative microscopic fields of BCAS1(green)/OLIG2 (red) positive cells in the PFC and STP from GW23 of control and DS. Blue, DAPI. Scale bar, 100  μm (low magnification), 20  μm (high magnification). White arrowheads indicate BCAS1/OLIG2 positive cells, and white dashed boxes indicate the interest area. e Quantification of BCAS1 (green)/OLIG2 (red) double-positive cells in PFC and STP from Control and DS group. n = 3 biological replicates per group. Two-sided paired Student’s t-test. Data are shown as mean ± SEM. Source data are provided as a Source Data file. *p < 0.05. PFC_DS vs Con: 95% confidence interval −10.32 to −1.541. STP_DS vs Con: 95% confidence interval −4.703 to −0.5140. f Visualization of Astro subclusters using UMAP. g Dot plot showing the average expression of the marker gene of Astro subclusters. h Bar plot showing the proportions of Astro subclusters in the control and DS from PFC and STP. i Representative microscopic fields of ID2 (green)/GFAP (red) positive cells in the PFC and STP from GW23 of control and DS. Blue, DAPI. Scale bar, 100 μm (low magnification), 20  μm (high magnification). White arrowheads indicate ID2/GFAP positive cells, and white dashed boxes indicate the interest area. j Quantification of ID2 (green)/GFAP (red) double-positive cells in PFC and STP from Control and DS group. n = 3 biological replicates per group. Two-sided paired Student’s t-test. Data are shown as mean ± SEM. Source data are provided as a Source Data file. *p < 0.05. PFC_DS vs Con: 95% confidence interval 8.357 to 37.39. STP_DS vs Con: 95% confidence interval 6.803 to 40.69. Heatmap shows the potential regulation target genes of the ExNs by ligands from OPC (k) and Astro (l). m OPC and Astro, respectively, regulate the development and positioning of neurons in PFC and STP through tubulin and DSCAM. Source data are provided as a Source Data file.

Back to article page