Fig. 1: The N-terminal parts of the IDs of RLP23 and RLP42 are predicted to adopt a β-strand conformation and interact through a β-strand with their respective ligands.

a AF3 predicts a high interface predicted Template Modeling (ipTM) for RLP23 and RLP42 in complex with their respective ligand and the co-receptor BAK1 (tripartite). AF3 guidelines state that ipTM values higher than 0.8 represent confident high-quality predictions. ipTM values between 0.6 and 0.8 are within a gray zone where predictions could be correct or incorrect. The AF3 cutoffs are depicted with a red and black dotted line. b, c Structural representations of the tripartite complexes of RLP23-nlp20-BAK1 and RLP42-pg13-BAK1. The ID is highlighted in black, other LRR-RP domains are depicted in dark gray. The ligand is depicted in yellow, labeled residues were earlier shown to be essential for functionality from the ligand side. Highlighted residues in light gray were mutagenized from the receptor side in this study^9,26. Pdb files can be found in Supplementary Data 1 and the predicted local distance difference test (pLDDT) scores can be found in Supplementary Fig. 2.