Fig. 3: Substrate-free state structures of LptB2FGC. | Nature Communications

Fig. 3: Substrate-free state structures of LptB2FGC.

From: Structural snapshots of Pseudomonas aeruginosa LptB2FG and LptB2FGC reveal insights into lipopolysaccharide recognition and transport

Fig. 3: Substrate-free state structures of LptB2FGC.

a Cryo-EM structure of LptB2FGC showing two different conformations. b Overlay of the LptB2FGC structures, showing rigid body movements in the periplasmic portions of the complex. Form I is shown in yellow and form II in purple, highlighting the conformational changes in the periplasmic regions of the transporter. (right) Zoomed-in view of the β-jellyroll domains, illustrating clockwise rotation toward the outer leaflet of the inner membrane. (bottom) Top view of the β-jellyroll domains, showcasing the overall conformational movement. c Overall structure of the LptB2FGC complex, highlighting the interaction between LptC and LptG. A loop region in LptG (residues 209–215) directly contacts the N-terminal jellyroll domain of LptC (residues 60–69). Close-up view of the LptC–LptG interface, showing residues within 3.5 Å.

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