Fig. 4: Melanogenic activity requires Treg-PPARγ signalling axis.
From: Early skin seeding regulatory T cells modulate PPARγ-dependent skin pigmentation
A Schematic timeline of PPARγ modulation experiment. Control group received DMSO on P6 and P8. ΔΕTreg group received DT and DMSO on P6 and P8. T0070907 group received the 2.5 µg/g of PPARγ antagonist, T0070907, on P6 and P8. ΔΕTreg GW1929 group received DT and 20 µg/g of GW1929 on P6 and P8. (NIAID Visual & Medical Arts. (10/7/2024). Lab Mouse. NIAID NIH BIOART Source. bioart.niaid.nih.gov/bioart/281). B Fontana & Masson staining. 250 µm scale bars. C Quantification of melanin area in stage-matched hair follicles. D–G Flow cytometric characterisation of MeSCs on P28. D, E Absolute number and percentage of Ki67+ MeSC upon antagonisation of PPARγ pathway. F, G Absolute number and percentage of Ki67+ MeSCs upon agonisation of PPARγ pathway in ETreg-depleted skin (ΔETreg). H, I Melanin area in Pparg-sufficient control (K14-PpargWT) and upon HF-specific deletion of Pparg (K14-PpargKO). 500 µm scale bars. C, F, G One way ANOVA. D, E, I Unpaired t-test. Graphs show mean ± S.E.M. Data are pooled from 2 independent experiments (n = 4–6 biological replicates). ***p < 0.001, **p < 0.01, *p < 0.05, ns p > 0.05. Source data are provided as a Source Data file.
