Fig. 5: Neonatal Tregs regulate the hair follicle transcriptome and PPARγ activity.
From: Early skin seeding regulatory T cells modulate PPARγ-dependent skin pigmentation
A UMAP representation of CD45- non-immune cells. B Ratio of non-immune cells in control and ΔETreg skin. Epithelia epithelial cells, Fib fibroblast, HF hair follicle, Mel melanocyte, Misc miscellaneous, Vasc vascular cell. C Number of differentially expressed genes (DEG) by cell type. D UMAP representation showing PPARγ score. E PPARγ score by cell type between control and ΔETreg skin. n = 3 biological replicates per condition. F Venn diagram depicting the overlap between the total differentially expressed genes (DEGs) and known PPARγ target genes. P value represents the significance of the overlap as determined by a chi-square test. G Volcano plot of representative differentially expressed PPARγ target genes, Apoe, Pltp, Igf1, and Abca1 (blue). H Cellchat analysis displaying interactions between cell types (nodes) and the cell-cell interactions (lines). I Melan-a cell line cultured in the absence (control) or presence of IGF-1 (100 ng/ml). Scale bar represents 50 μm. J Quantification of melanin area. 6 technical replicates per condition. Two-sided unpaired t-test. Graphs show mean ± S.E.M. K NicheNet analysis using HF cells subsetted from mouse scRNA-seq and sorted Tregs from P9 skin (NIAID Visual & Medical Arts. (10/7/2024). T Cell. NIAID NIH BIOART Source. bioart.niaid.nih.gov/bioart/508). L Volcano plot showing higher expression of Il10 and Tnfrsf9 in skin Tregs than in SDLN Tregs. Source data are provided as a Source Data file.
